ABSTRACT
Object The permeability of integerrimine was studied in vitro to design its anticancer preparations Methods Vilia Chien diffusion cells were adopted as apparatus for in vitro skin permeation, nude mouse skins were used as permeation barrier and permeation coefficient (P) was calculated The concentrations of integerrimine in samples were measured by RP HPLC, and the effects of Azone, 4% Tween 80, 8% propylene glycol on it were studied Results Its P is 1 184?10 -2 cm/h with water solution as donor and pH 6 8 PBS as receptor Its enhancement rate (ER) of 2% Azone and 8% propylene glycol is 2 8 and 1 5, while Tween 80 inhibits its penetration Conclusion Integerrimine is a good candidate of antiskin cancer for transdermal drug delivery, and the optimal formulation can be designed according to the experiments
ABSTRACT
Objective To study the antitumor activities of extract from Ligia exotica(Roux).Methods The dried powder of total Ligia exotica(Roux) was extracted by 37 ℃ water.The solution was concentrated in vacuum,and then was freeze-dried to afford crude extract.The inhibitory effect of the extract on tumor cells proliferation was assayed by MTT method,and transplant tumor model of sarcoma 180(S180) was used.Results The extract from Ligia exotica displayed obvious proliferation inhibitory effect on HeLa,7901,NCI cells,and no growth inhibitory effect on 929 cells in vitro.After administration at the doses of 0.25,0.50,1.00 g?kg-1,ip,for 7d in tumor-bearing mice with S180,The extract caused 26.9 %,45.3 %,64.6 % inhibition rates,respectively.Conclusion The extract from Ligia exotica showed significant antitumor activity.
ABSTRACT
AIM: The gel vehicle was optimized by release and transdermal resorption of integerrimine in vitro in order to design its anti-cancer transdermal drug delivery system. METHODS: The releasing rate was detected by dissolution Vilia-Chien diffusion cells, nude mouse skin were used as permeation barrier, the concentration of integerrimine in samples was measured by RP-HPLC. RESULTS: Integerrimine releases of three different vehicles conformed to Higuchi equation, the releasing rate of CMC-Na gel is faster than that of HPMC gel, and that of Carbopol gel is the slowest in three, and corresponded with zero kinetic equation. CONCLUSION: HPMC is an drug vehicle of choice.